Quantitative analysis of cholesterol in foods by gas-liquid chromatography.

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Abstract
To develop a simple and exact analytical method for cholesterol determination in [animal] foods by GLC, several experiments were carried out in collaboration with several universities and institutes. For the extraction of lipids from foods, chloroform-methanol (2:1) extraction was the most suitable procedure. Pretreatment procedures using methods such as TLC and florisil column chromatography to purify the unsaponifiable matters reduced the recovery of cholesterol but good results were obtained without applying the pretreatment; these procedures were unnecessary. Gas chromatograms obtained with free sterol showed results similar to those of the trimethylsilyl ether derivative and acetate. 5-.alpha.-Cholestane is used as a good internal standard.

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