DECONTAMINATION OF CADAVER CORNEAS

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Abstract
Bacterial contamination of human corneas, in vivo and post mortem, was investigated. Bacterial samples were obtained by pressing small concave agar plates directly against the corneas. More colony forming units (CFU) occurred in samples obtained from patients in general anesthesia than in samples obtained from healthy persons in local anesthesia. The largest number of CFU occurred post mortem. The number of CFU obtained from cadaver corneas was not correlated to post mortem time. Rinsing with water removed 86% of the CFU on cadaver corneas. Rinsing with water for 30 s followed by 2 min treatment with 0.5% polyvinylpyrrolidone-iodine (PVP-I) and repeated rinsing removed 98% of the CFU. Epithelial or stromal lesions were not found after 2 min of exposure to 0.5% PVP-I.

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