Separation of active and inactive (nonphosphorylating) Ca2+‐ATPase in sacroplasmic reticulum subfractions from low‐frequency‐stimulated rabbit muscle
- 9 December 1991
- journal article
- Published by Wiley in FEBS Letters
- Vol. 294 (3) , 203-206
- https://doi.org/10.1016/0014-5793(91)80669-t
Abstract
Chronic low‐frequency stimulation elicits in rabbit fast‐twitch muscle a partial inactivation of the sarcoplasmic reticulum (SR) Ca2+‐ATPase and Ca2+‐uptake activities. Inactive Ca2+‐ATPase was enriched in a light microsomal fraction by sucrose density gradient centrifugation after calcium oxalate loading in the presence of ATP. This fraction showed a reduced specific activity and phosphoprotein formation of the Ca2+‐transport ATPase. These results suggest that the inactivation of the Ca2+‐ATPase as induced by increased contractile activity, is confined to a specific SR vesicle population.Keywords
This publication has 15 references indexed in Scilit:
- Chronic low‐frequency stimulation of rabbit fast‐twitch muscle induces partial inactivation of the sarcoplasmic reticulum Ca2+‐ATPase and changes in its tryptic cleavageEuropean Journal of Biochemistry, 1990
- Slow/cardiac sarcoplasmic reticulum Ca2+‐ATPase and phospholamban mRNAs are expressed in chronically stimulated rabbit fast‐twitch muscleEuropean Journal of Biochemistry, 1989
- Relations between chronic stimulation-induced changes in contractile properties and the Ca2+-sequestering system of rat and rabbit fast-twitch musclesPflügers Archiv - European Journal of Physiology, 1989
- Characterization of membrane calcium pumps by simultaneous immunoblotting and 32P radiographyBiochimica et Biophysica Acta (BBA) - Biomembranes, 1988
- A monoclonal antibody to the Ca2+ ‐ATPase of cardiac sarcoplasmic reticulum cross‐reacts with slow type I but not with fast type II canine skeletal muscle fibers: An immunocytochemical and immunochemical studyCell Motility, 1988
- Reversible inhibition of sarcoplasmic reticulum Ca‐ATPase by altered neuromuscular activity in rabbit fast‐twitch muscleEuropean Journal of Biochemistry, 1987
- Two telestimulation systems for chronic indirect muscle stimulation in caged rabbits and micePflügers Archiv - European Journal of Physiology, 1983
- Molecular Transformations in Sarcolasmic Reticulum of Fast‐Twitch Muscle by Electro‐StimulationEuropean Journal of Biochemistry, 1979
- ATPase Activities, Ca2+ Transport and Phosphoprotein Formation in Sarcoplasmic Reticulum Subfractions of Fast and Slow Rabbit MusclesEuropean Journal of Biochemistry, 1977
- Effects of long-term electrical stimulation on some contractile and metabolic characteristics of fast rabbit musclesPflügers Archiv - European Journal of Physiology, 1973