Fetal and Newborn Calf Thymus as a Source of Chromatin Proteins: Purification of HMG-1 and HMG-2
- 1 March 1987
- journal article
- research article
- Published by Taylor & Francis in Preparative Biochemistry
- Vol. 17 (1) , 51-61
- https://doi.org/10.1080/00327488708062476
Abstract
The high mobility group (HMG) non-histone chromosomal proteins were first isolated from calf thymus' but were later found in numerous organs of many vertebrates.' The proteins can be extracted from calf thymus 1 with 0.35 M NaCl and they are quite soluble in 2% trichloroacetic acid. We have shown that members of the HMG-1 family (i.e., HMG-1, HMG-2, and HMG-E) exhibit a preferential affinity for single-stranded DNA at roughly physiological ionic trength. Members of this family have other intriguing properties (see references 6 and 7 for recent reviews), including the ability to assemble nucleosomes in vitroe8 The architecture of the proteins strongly suggests that they are designed to interact simultaneously with histones and with DNA through physically distinct domains6, 9.This publication has 13 references indexed in Scilit:
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- Comparison of the structures of the chromosomal high mobility group proteins HMG1 and HMG2 prepared under conditions of neutral and acidic pHBiochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1983
- Domain structure in high molecular weight high mobility group nonhistone chromatin proteinsNature, 1982
- Removal of degradation products from calf thymus high mobility group non-histone chromatin proteins by chromatography on immobilized double-stranded DNABiochimica et Biophysica Acta (BBA) - Gene Structure and Expression, 1982
- Species and Tissue SpecificityPublished by Elsevier ,1982
- The primary structures of non‐histone chromosomal proteins HMG 1 and 2FEBS Letters, 1980
- Isolation and separation of chicken erythrocyte high mobility group non‐histone chromatin proteins by chromatography on phosphocelluloseFEBS Letters, 1980
- Purification from cultured hepatoma cells of two nonhistone chromatin proteins with preferential affinity for single-stranded DNA: Apparent analogy with calf thymus HMG proteinsBiochemical and Biophysical Research Communications, 1978
- A New Group of Chromatin‐Associated Proteins with a High Content of Acidic and Basic Amino AcidsEuropean Journal of Biochemistry, 1973