Rapid synthesis of oligodeoxyribonucleotides III. Effect of added carboxylate ion on the efficiency of internucleotide bond formation. Solid-phase synthesis of the dodecanucleotide, d(pT-A-A-C-T-G-C-T-C-A-C-T)
- 1 January 1979
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 6 (4) , 1259-1268
- https://doi.org/10.1093/nar/6.4.1259
Abstract
In solid-phase oligodeoxyribonucleotide synthesis the addition of benzoic acid to internucleotide phosphodiester bond-forming reactions lowers coupling yields by far greater amount than anticipated on a simple nucleotide-carboxylate molar ratio. High coupling yields were consistently obtained in synthesis of the dodecanucleotide, d(pT-A-A-C-T-G-C-T-C-A-C-T), using rigorously purified nucleotide starting materials.Keywords
This publication has 4 references indexed in Scilit:
- Complete sequence of constant and 3′ noncoding regions of an immunoglobulin mRNA using the dideoxynucleotide method of RNA sequencingCell, 1978
- Studies on polynucleotides. 146. High-pressure liquid chromatography in polynucleotide synthesisBiochemistry, 1978
- Rapid synthesis of oligodeoxyribonucleotides: a new solid-phase methodNucleic Acids Research, 1977
- Rapid synthesis of oligodeoxyribonucleotides II1. Machine-aided solid-phase syntheses of two nonanucleotides and an octanucleotideNucleic Acids Research, 1977