Effects of Cisplatin on Cell Cycle Kinetics, Morphological Change, and Cleavage Pattern of DNA in Two Human Ovarian Carcinoma Cell Lines
- 1 January 1996
- journal article
- research article
- Published by S. Karger AG in Oncology
- Vol. 53 (1) , 19-26
- https://doi.org/10.1159/000227529
Abstract
We evaluated the effects of cisplatin on the cell kinetics, cytomorphological changes, and cleavage patterns of DNA in two lines of human ovarian carcinoma cells. KF-1 cells displayed a cell cycle arrest in the G2M phase, while HMG cells displayed a transient cell accumulation in the S phase, without obvious G2M arrest. Morphological changes characterized by condensation and fragmentation of chromatin, and DNA cleavage by oligonucleosome-sized DNA fragments were observed in the HMG cells but not in the KF-1 cells. The pattern of cell death in HMG cells was considered to be apoptosis, but that of KF-1 cells necrosis. These findings showed the different mechanisms of antitumor effect of cisplatin on human ovarian carcinoma cell lines, including cell kinetics and pattern of cell death.Keywords
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