NF‐κB elements contribute to junB inducibility by lipopolysaccharide in the murine macrophage cell line RAW264.7
- 25 January 2002
- journal article
- Published by Wiley in FEBS Letters
- Vol. 513 (2-3) , 203-207
- https://doi.org/10.1016/s0014-5793(02)02295-0
Abstract
Macrophages respond to bacterial lipopolysaccharide (LPS) by activating latent cis-acting factors that initiate transcription of immediate early genes. One such immediate early gene, junB, is induced by LPS in macrophages within 30 min. To identify elements that mediate the induction of junB by LPS, upstream and downstream sequences flanking the junB gene were examined by transient expression in the RAW264.7 murine macrophage cell line using a luciferase reporter gene vector containing the junB minimal promoter. A >10-fold enhancement was associated with a 222 bp region downstream of the junB promoter in response to LPS. Transient reporter assays demonstrated that multiple nuclear factor (NF) κB sites are required for inducibility of junB by LPS in RAW264.7 cells. Electrophoretic mobility shift assays confirmed binding of LPS-induced nuclear proteins included p50/p65 heterodimers at these NF-κB sites.Keywords
This publication has 17 references indexed in Scilit:
- Evaluation of Methods for Transient Transfection of a Murine Macrophage Cell Line, RAW 264.7BioTechniques, 1999
- IFN-gamma and IL-10 inhibit induction of IL-1 receptor type I and type II gene expression by IL-4 and IL-13 in human monocytes.The Journal of Immunology, 1997
- Oxidized low density lipoprotein inhibits lipopolysaccharide-induced binding of nuclear factor-kappaB to DNA and the subsequent expression of tumor necrosis factor-alpha and interleukin-1beta in macrophages.Journal of Clinical Investigation, 1996
- Control of IκB-α Proteolysis by Site-Specific, Signal-Induced PhosphorylationScience, 1995
- Concerted Participation of NF-κB and C/EBP Heteromer in Lipopolysaccharide Induction of Serum Amyloid A Gene Expression in LiverPublished by Elsevier ,1995
- Multiple regulatory elements in the interleukin-6 gene mediate induction by prostaglandins, cyclic AMP, and lipopolysaccharide.Molecular and Cellular Biology, 1994
- Three NF-χB sites in the IχB-α promoter are required for induction of gene expression by TNFαNucleic Acids Research, 1994
- Selection of optimal kappa B/Rel DNA-binding motifs: interaction of both subunits of NF-kappa B with DNA is required for transcriptional activation.Molecular and Cellular Biology, 1992
- Interleukin-6 signals activating junB and TIS11 gene transcription in a B-cell hybridoma.Molecular and Cellular Biology, 1991
- DNA binding activities of three murine Jun proteins: Stimulation by FosCell, 1988