Effect of transforming growth factor β on fibroblasts in three‐dimensional lattice cultures
- 26 March 1990
- journal article
- Published by Wiley in FEBS Letters
- Vol. 262 (2) , 339-341
- https://doi.org/10.1016/0014-5793(90)80223-6
Abstract
Human skin fibroblasts were cultivated in three-dimensional fibrin or collagen lattices, under retracting or non-retracting conditions, and the influence of transforming growth factor β (TGFβ) was tested. TGFβ stimulated the synthesis of non-collagen protein and of collagen in all the systems. However, only in non-retracting fibrin lattices did it restore a level of protein synthesis comparable to that found in monolayers. The effects of TGFβ greatly depended on the type of substratum and on the presence or absence of retraction.Keywords
This publication has 16 references indexed in Scilit:
- Cultures of fibroblasts in fibrin lattices: Models for the study of metabolic activities of the cells in physiological conditionsJournal of Cellular Physiology, 1989
- Growth Rate of Human Fibroblasts is Repressed by the Culture within Reconstituted Collagen Matrix but not by the Culture on the MatrixMatrix, 1989
- Transforming growth factor β stimulates collagen and glycosaminoglycan biosynthesis in cultured rabbit articular chondrocytesFEBS Letters, 1988
- Differential effects of transforming growth factor-beta on the synthesis of extracellular matrix proteins by normal fetal rat calvarial bone cell populations.The Journal of cell biology, 1988
- Transforming growth factor beta increases mRNA for matrix proteins both in the presence and in the absence of changes in mRNA stability.Proceedings of the National Academy of Sciences, 1988
- Fibronectin dependence of the contraction of collagen lattices by human skin fibroblastsExperimental Cell Research, 1986
- Collagen Biosynthesis by Cells in a Tissue Equivalent Matrix In VitroCollagen and Related Research, 1984
- Regulation of proliferation of fibroblasts of low and high population doubling levels grown in collagen latticesMechanisms of Ageing and Development, 1981
- Rates of DNA and protein syntheses by fibroblast cultures in the presence of various glucose concentrationsBiochimie, 1980
- Use of a mixture of proteinase-free collagenases for the specific assay of radioactive collagen in the presence of other proteinsBiochemistry, 1971