Possibility of Bacteroides gingivalis Hemagglutinin Possessing Protease Activity Revealed by Inhibition Studies
- 1 January 1989
- journal article
- research article
- Published by Wiley in Microbiology and Immunology
- Vol. 33 (1) , 75-80
- https://doi.org/10.1111/j.1348-0421.1989.tb01499.x
Abstract
Inhibition of hemagglutinin (HA) activity in a membrane fraction of Bacteroides gingivalis was examined using various compounds. Leupeptin and antipain inhibited the HA activity at nm order. This potency was lost when the aldehyde group of leupeptin was converted to an alcohol moiety. Irreversible protease inhibitors, tosyl‐l‐lysine chloromethyl ketone (TLCK), p‐chloromercuribenzoate (PCMB), and N‐ethylmaleimide (NEM) were also inhibitory. From the inhibition experiments, we speculate that the HA possesses protease activity and that the same site of the molecule participates in the erythrocyte binding and the substrate binding.This publication has 15 references indexed in Scilit:
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