Isolation of Mumps Virus from the Blood of a Patient

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Abstract
Blood was drawn from a patient on the day of onset of parotitis and inoculated into the amniotic sac of 7-day-old embry-onated eggs. The eggs were incubated for 6 days, at which time the amniotic membranes and fluids were harvested. Mumps virus appeared on the 3d egg passage as indicated by the macroscopic hemagglutination of hen erythrocytes. Using paired sera known to show a rise in titer of mumps antibodies, the virus was identified by the inhibition of hemagglutination and by a complement-fixation test. Using the Enders strain of mumps virus as antigen, the patient was proven to have mumps serologically by the rise in titer in the complement-fixation and in the inhibition of hemagglutination tests. Mumps virus was also recovered from 5 to 7 cerebrospinal fluids of patients with proven mumps meningo-encephalitis.
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