RAPID RADIOIMMUNOASSAY OF PROGESTERONE IN UNEXTRACTED BOVINE PLASMA

  • 1 January 1978
    • journal article
    • research article
    • Vol. 42  (2) , 229-234
Abstract
A rapid radioimmunoassay for determining progesterone levels in unextracted bovine plasma was developed. Two antisera were tested, anti-deoxycorticosterone-21-hemisuccinate human serum albumin and anti-progesterone-11-hemisuccinate bovine serum albumin. The usefulness of an antiserum for rapid assays was based on its agreement with assays of organic extracts or chromatographically purified extracts of selected bovine samples. To avoid large blank effects and nonspecific interference, a plasma volume of 20 .mu.l that provided adequate sensitivity was not exceeded. All correlations between rapid assays, assays using organic extraction or assays after chromatographic purification were greater than 0.9 and showed regression slopes approximating unity. Individual lots of antisera should be evaluated thoroughly before use in a rapid assay.