Measurement of eight prostaglandins in human gingival and periodontal disease using high pressure liquid chromatography and radioimmunoassay

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Abstract
Eight prostaglandins (PG) of biological importance, namely PGE,. PGE1 13,14‐dihydro‐15‐keto‐PGE, (DHK‐PGE2), PGF2α, PGF1α, 13, 14‐dihydro‐15‐keto‐PGF2α (DHK‐PGF2α, 6‐keto‐PGF1α, and thromboxane B2 (TxB2), were measured in the human gingiva in a healthy state, gingivitis. and periodontitis. High pressure liquid chromatography (HPLC) in combination with radioimmunoassay (RIA) was employed for quantitative analysis. The advantages of HPLC over the classic silicic acid column chromatography of prostaglandins were discussed. All prostaglandins considered were shown to increase during advancing periodontal destruction. The most quantitatively significant endoperoxide metabolites at every stage of inflammation were TxB2 and 6‐keto‐PGF1α followed by PGE2. DHIK‐PGE2, PGE1, PGF1α. PGF3α, and DHK‐PGF2α in decreasing order.