Two Glycosulfatases from the Liver of a Marine Gastropod, Charonia lampas Partial Purification and Properties1
- 1 January 1976
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Biochemistry
- Vol. 79 (1) , 27-34
- https://doi.org/10.1093/oxfordjournals.jbchem.a131054
Abstract
Two glycosulfatases [EC 3.1.6.3], I and II, were purified 31.3- and 33.9-fold respectively, from a crude extract of the liver of Charonia lampas. The purification was carried out by the following chromatographic procedures; phosphocellulose, Sephadex G-150, Concanavalin A-Sepharose and isoelectric focussing. The enzyme preparations obtained were practically free from arylsulfatase [EC 3.1.6.1] contamination. Both glycosulfatases are probably glycoproteins differing in their carbohydrate moieties. The molecular weights of glycosulfatase I and II were estimated to be about 112, 000 and 79, 000, respectively. They had the same optimum pH of 5.5, and the same Km value of 25.0 mM for glucose 6-sulfate.Keywords
This publication has 1 reference indexed in Scilit:
- The gel-filtration behaviour of proteins related to their molecular weights over a wide rangeBiochemical Journal, 1965