Effect of growth hormone on ribonucleic acid metabolism. 1. Incorporation of radioactive phosphate into ribonucleic acid fractions of rat liver

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Abstract
The effects of growth-hormone treatment and of partial hepatectomy on the incorporation of labelled orthophosphate into ribonucleic acid from several subcellular fractions (nuclear soluble, nucleolar, cytoplasmic soluble and microsomal) of rat liver have been studied. The specific activity of P32 increased in all fractions of liver ribonucleic acid on growth-hormone treatment, but the increase in nucleolar and microsomal fractions is significantly marked. In regenerating liver there was a uniform increase in specific activity of P32 in all fractions. On growth-hormone administration, a quantitative increase in the content of ribonucleic acid over the normal levels occurs only in the nucleolar fraction, the content of microsomal ribonucleic acid showing a decline. In regenerating liver, the major quantitative increase is in the nuclear fraction (nuclear soluble 49 3%, nucleolar 218*5%).