The determination of oestrogens in human pregnancy urine. A new method of correcting for the brown colour developed in the Kober reaction by non-oestrogenic substances

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Abstract
The pink solns. yielded by estrone or estriol in the Kober reaction with the phenolsulphonic acid reagent are completely decolorized after heating at 100[degree] for 1.5 hr. The brown solns. yielded by ether-soluble phenolic fractions of human [male] urine in the Kober reaction undergo little change, as judged by absorption at c. 520 m[mu], when similarly treated. On the basis of these observations, a simple method has been developed of correcting for the interfering brown color which is developed from non-estrogenic substances in the detn. of estrogens in human pregnancy urine.