Microdetermination of adrenocortical steroids by double isotope method. V. Interaction between corticosteroids and human erythrocytes.

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Abstract
Interaction between corticosteroids and human erythrocytes, and the mode of incorporation of corticosteroids into erythrocytes were examined by electrophoresis, gel filtration, and other chromatographic methods, in combination with the double isotope derivative dilution method. Corticosteroids were incorporated into erythrocytes in physiological saline and subfractions of erythrocytes were separated and analyzed by the above methods. For example, cortisol was found to be bound or adsorbed on the erythrocyte surface layer to 38% (55.8% bound and 44.2% adsorbed), incorporated into the membrane to 1.7% (50% in free form, 40% bound to lipid, and 10% bound to protein), and 25.3% incorporated into the internal fluid of the cell (60% in free form and 40% bound to hemoglobin and other internal fluid components). These results indicated that a part of corticosteroids is adsorbed in the free form on the erythrocyte surface and also bound to sialoglycoprotein, and also incorporated into the membrane by binding with membrane components, lipid and protein. Further, the steroids are transported into the internal fluid by penetration through the membrane, a part of which are bound to hemoglobin and other internal fluid components and others are present in the free form. Thus, the passage of corticosteroids through the erythrocyte membrane is a passive and nonspecific transport, which, would be based on the diffusion restricted by a lipid barrier and diffusion facilitated by lipid and protein in the membrane as a carrier according to the classification of Park.

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