Phosphorylation-dephosphorylation differentially affects activities of splicing factor ASF/SF2
Open Access
- 2 November 1998
- journal article
- research article
- Published by Springer Nature in The EMBO Journal
- Vol. 17 (21) , 6359-6367
- https://doi.org/10.1093/emboj/17.21.6359
Abstract
SR proteins are a conserved family of splicing factors that function in both constitutive and activated splicing. We reported previously that phosphorylation of the SR protein ASF/SF2 enhances its interaction with the U1 snRNP‐specific 70K protein and is required for the protein to function in splicing, while other studies have provided evidence that subsequent dephosphorylation can also be required for SR protein function, at least in constitutive splicing. We now show that the phosphorylation status of ASF/SF2 can differentially affect several properties of the protein. In keeping with a dynamic cycle of phosphorylation–dephosphorylation during splicing, ASF/SF2 phosphorylation was found to affect interaction with several putative protein targets in different ways: positively, negatively or not at all. Extending these results, we also show that, in contrast to constitutive splicing, dephosphorylation is not required for ASF/SF2 to function as a splicing activator. We discuss these results with respect to the differential protein–protein interactions that must occur during constitutive and activated splicing.Keywords
This publication has 81 references indexed in Scilit:
- Dynamic relocation of transcription and splicing factors dependent upon transcriptional activityThe EMBO Journal, 1997
- Assembly of specific SR protein complexes on distinct regulatory elements of the Drosophila doublesex splicing enhancer.Genes & Development, 1996
- The SR protein family: pleiotropic functions in pre-mRNA splicingTrends in Biochemical Sciences, 1996
- The SR protein family: pleiotropic functions in pre-mRNA splicingTrends in Biochemical Sciences, 1996
- The splicing factor U2AF35 mediates critical protein-protein interactions in constitutive and enhancer-dependent splicing.Genes & Development, 1996
- Intron-dependent recruitment of pre-mRNA splicing factors to sites of transcription.The Journal of cell biology, 1996
- A U6 snRNA:pre-mRNA interaction can be rate-limiting for U1-independent splicing.Genes & Development, 1995
- A serine kinase regulates intracellular localization of splicing factors in the cell cycleNature, 1994
- Disruption of base pairing between the 5′ splice site and the 5′ end of U1 snRNA is required for spliceosome assemblyCell, 1993
- Thiophosphorylation of U1-70K protein inhibits pre-mRNA splicingNature, 1993