SEQUENTIAL BLOCKADE IN ADENINE BIOSYNTHESIS BY GENETIC LOSS OF AN APPARENT BIFUNCTIONAL DEACYLASE
- 15 September 1957
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 43 (9) , 826-834
- https://doi.org/10.1073/pnas.43.9.826
Abstract
Some adenine-requiring mutants of Escherichia coli and Salmonella typhimurium accumulate a precursor which has been identified as the ribotide of 5-amino-4-imidazole-N-succinylocarboxamide (S-AICAR). The riboside was also found. The accumulation is due to a mutational loss of a deacylating enzyme, which splits S-AICAR to yield the ribotide of 5-amino-4-imidazolecarboxyamide. This loss is accompanied by a loss of the ability to deacylate adenylo-succinic to adenylic acid, resulting in the specific requirement for adenine. Thus, the mutational loss of this apparent bifunctional deacylase creates a double block along the biosynthetlc pathway to nucleic acid adenine.This publication has 8 references indexed in Scilit:
- THE GENETIC CONTROL OF ADENYLOSUCCINASE IN Neurospora CrassaProceedings of the National Academy of Sciences, 1957
- Identification of major accumulation products of adenine-specific mutants of NeurosporaArchives of Biochemistry and Biophysics, 1957
- Accumulation of adenine-succinic acid by an adenine-requiring mutant of Neurospora crassaArchives of Biochemistry and Biophysics, 1956
- Enzymatic synthesis of adenosine-5'-phosphate from inosine-5'-phosphate.1956
- THE PREPARATION AND PROPERTIES OF ADENYLOSUCCINASE AND ADENYLOSUCCINIC ACIDJournal of Biological Chemistry, 1956
- PURINE METABOLISM IN BACTERIA .2. FACTORS INFLUENCING BIOSYNTHESIS OF 4-AMINO-5-IMIDAZOLECARBOXAMIDE BY ESCHERICHIA-COLI1954
- THE PURINE AND PYRIMIDINE METABOLISM OF NORMAL AND PHAGE-INFECTED ESCHERICHIA COLIJournal of Biological Chemistry, 1953
- IDENTIFICATION AND ESTIMATION OF PENTOSES IN THE PRESENCE OF GLUCOSE1948