Soluble Protective Antigen from Bordetella Pertussis Prepared with Sodium Desoxycholate

Open Access
Publisher Website
Google Scholar
Abstract
Summary: Treatment of Bordetella pertussis with sodium desoxycholate caused lysis of cells and liberation of the mouse protective antigen in soluble form and with a good yield. Optimum conditions for solubilization of protective antigen were 0.5 to 1% desoxycholate, pH 8.5, 0 to 2°C and a reduction in optical density of 40 to 50%. Approximately 75% of the bacterial dry weight was dissolved under these conditions. However, the soluble fraction was virtually nontoxic in contrast to ultrasonic extracts of the same cells. Millipore filtration was an effective method of sterilizing solutions of protective antigen.