ON THE FORMATION OF PROTOPLAST-LIKE STRUCTURES FROM STREPTOMYCETES

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Abstract
Osmotically sensitive protoplast-like structures were produced by the action of lysozyme on streptomycete mycelium suspended in a buffered sucrose medium. A comparison was made of the surface of the mycelium and that of the "protoplasts" by virus adsorption and serological techniques. Although the "protoplasts" did not possess the phage receptor sites present on the original hyphae they did contain antigens in common with the cell walls. These data indicated that the structures did not have properties exactly comparable to those which have been described for protoplasts from Bacillus megaterium.