Comparison of Mobile Phases for Separation of Phospholipids by One-Dimensional TLC on Preadsorbent High Performance Silica Gel Plates
- 1 November 1991
- journal article
- research article
- Published by Taylor & Francis in Journal of Liquid Chromatography & Related Technologies
- Vol. 14 (18) , 3269-3275
- https://doi.org/10.1080/01483919108049388
Abstract
Eight solvent systems reported in the literature for the one-dimensional TLC separation of phospholipids were compared under identical conditions using high performance preadsorbent silica gel plates. The best overall separation of phospholipid standards was obtained by a single development with chloroform-methanol-water (65:25:4), and three other systems containing chloroform also gave good separations. Rf data are tabulated for these four systems, and the phospholipids extracted from the digestive gland-gonad complex of Biomphalaria glabrata snails are identified.Keywords
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