Immunoperoxidase Identification of Intracellular Immunoglobulins from Cell Smears

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Abstract
Optimal fixation procedures for intracellular immunoglobulins (Ig) were defined by using cytocentrifuged or conventional cell smears of pokeweed-mitogen-stimulated human lymphocytes. The Ig was detected by the unlabeledantibody- enzyme technic. The results indicate that superior preservation of Ig antigenicity combined with good morphologic integrity is obtained with 2–4 min fixation in Baker’s formolcalcium at 20 C with subsequent wash in distilled water. This technic is currently used for the detection of Ig isotypes from bone marrow aspirates and from B cell mitogen-stimulated cell cultures.