Cre‐mediated recombination in rhombic lip derivatives
- 6 August 2002
- Vol. 33 (4) , 160-169
- https://doi.org/10.1002/gene.10104
Abstract
Summary: To study the development of the cerebellum, we generated a transgenic mouse line Tg(mα6‐cre)B1LFR that expresses CRE recombinase under the GABAA receptor α6 subunit promoter. In this line, recombination of an R26R reporter allele occurred postnatally in granule cells of the cerebellum and dorsal cochlear nucleus, as well as in a subset of precerebellar nuclei in the brainstem. All neurons in which recombination occurred originated during embryogenesis from the rhombic lip. This might be explained by a very early specification event at the rhombic lip that primes cells derived from this structure to express the transgene during neuronal maturation. As no recombination occurred in the inferior olive, it may be derived from a distinct subset of precursors at the rhombic lip. No recombination occurred in any of the interneurons in the cerebellum (stellate cells, basket cells, and Golgi cells), consistent with the hypothesis that they are not derived from the same embryonic tissue as the granule cells. genesis 33:160–169, 2002.Keywords
Funding Information
- U.S. Public Health Service (NS16033)
- the Swiss National Science Foundation
- Uarda Frutiger Foundation
- Howard Hughes Medical Institute
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