An Improved Method for Double-Isotope and Double-Emulsion Radioautography Using Epoxy Resin Sections

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Abstract
This technique for the quantitation of silver grains in radioautographs produced by two differently labeled precursors of proteins and ribonucleic acids involves the use of 0.5 μ-thick sections from as many as 24 different blocks of tissue on a single microscope slide. Thereby, the incorporation of uridine-3H and leucine-14C by exocrine cells of the pancreas and major salivary glands was studied. The results indicate: (1) that this technique can be applied successfully in a simultaneous evaluation of two metabolic aspects in a given population of cells, and (2) that standardization of the mounting procedure permits multiple statistical comparisons of different organs.