Isolation of Acholeplasma laidlawii from Commercial, Serum-Free Tissue Culture Medium and Studies on Its Survival and Detection
- 1 January 1974
- journal article
- research article
- Published by American Society for Microbiology in Applied Microbiology
- Vol. 27 (6) , 1046-1052
- https://doi.org/10.1128/aem.27.6.1046-1052.1974
Abstract
This report documents the first isolation of Acholeplasma laidlawii from a commercial lot of serum-free Dulbecco basal medium. Experimental studies demonstrated survival of the organism for at least 1 year depending on the concentration of the contaminating organism as well as pH and temperature of storage of the serum-free medium. A comparison of isolation methods showed that concentration by filtration through 220-nm membrane filters and testing the filters for mycoplasma recovery, especially when using phenol red-diphasic Hayflick medium, was both sensitive and practical for the average laboratory.Keywords
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