Stimulation of glucose uptake by transforming growth factor beta: evidence for the requirement of epidermal growth factor-receptor activation.

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Abstract
Transforming growth factor .beta. (TGF-.beta.), derived from human platelets, stimulates the uptake of 2-deoxyglucose by cultured cell monolayers 2- 4-fold. Stimulation can be detected as early as 30 min with as little as 0.1 ng of TGF-.beta. per ml and maximal effects can be obtained at 2 h with 1 ng of the growth of factor per ml. TGF-.beta.-induced stimulation of sugar uptake is enhanced by the co-addition of platelet derived growth factor (10 ng/ml) or epidermal growth factor (EGF, 1 ng/ml). The NR-6 variant of mouse 3T3 cells, which lack EGF receptors, is not stimulated by TGF-.beta.. Antisera to EGF receptors that block 125I-labeled ECG binding also inhibit TGF-.beta. stimulation of 2-deoxyglucose uptake. Although 125I-labeled TGF-.beta. binding remains unimpaired. Anti-sera to the EGF receptor, which do not block EGF binding, have no measurable effect on the TGF-.beta.-stimulated uptake of 2-deoxyglucose. The receptor for TGF-.beta. is distinct from the receptor for EGF and TGF-.beta. stimulation of 2-deoxyglucose uptake requires the co-activation of the EGF receptor kinase system.