OBSERVATIONS ON THE FLUORESCENCE, BIREFRINGENCE AND HISTOCHEMISTRY OF THE RAT OVARY DURING THE REPRODUCTIVE CYCLE1

Abstract
Sections from rat ovaries removed at different known stages of the reproductive cycle were examined to determine the localization of birefringent crystals, auto-fluorescent materials, acetone-soluble keto-compounds, and sulfuric acid positive sterol substances. By means of these tests, substances which gave the characteristic reactions of sterol hormones were detected in the theca interna but not in the granulosa. These substances were also present in the corpus luteum and in the interstitial tissue. The amts. of the reacting substances in the several structures varied according to the stage of the reproductive cycle.