Ontogeny of 3H-Diazepam Binding Sites in Different Rat Brain Area

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Abstract
The ontogenesis of the 3H-diazepam binding sites and their modulation by gamma-aminobutyric acid have been studied in different brain areas of rat at various ages, using frozen and two Triton X-100 treated crude synaptic membrane preparations. Benzodiazepine recognition sites are present at birth in all the brain regions investigated and reach adult levels about 3 weeks later. The changes of 3H-diazepam binding with age are due to an increase in the total number of binding sites. In vitro addition of GAB A produces, in newborn and adult cerebral cortex, an increase in affinity, but not in number of binding sites for 3H-diazepam. The effect is age dependent.