Versuche zur Anreicherung und Solubilisierung des muscarinischen Acetylcholinreceptors aus dem Nucleus caudatus vom Rind/Attempts to Enrich and to Solubilize the Muscarinic Acetylcholinereceptor from Bovine Caudate nucleus
Open Access
- 1 February 1979
- journal article
- Published by Walter de Gruyter GmbH in Zeitschrift für Naturforschung C
- Vol. 34 (1-2) , 51-59
- https://doi.org/10.1515/znc-1979-1-213
Abstract
Neuronal membranes of postsynaptic origin twofold enriched in acetylcholinesterase, muscarinic acetylcholinereceptor and (Na+/K+)-ATP-Phosphohydrolase, proteins associated with cholinergic nerve excitability, were prepared with yields between 60 and 75% from bovine caudate nucleus. On subfractionation of these membranes an additional twofold enrichment of the mentioned proteins is achieved in different subfractions. SDS-gradient gel electrophoresis shows that these subfractions have slightly different polypeptide compositions. Neuronal membranes of presynaptic origin on the other hand, prepared from purified synapto-somes, possess only small amounts of the mentioned proteins, showing no enrichment with respect to the homogenate. Solubilization of acetylcholinesterase with 1 ᴍ NaCl as well as of muscarinic acetylcholinreceptor with 2 ᴍ NaCl does not succeed. These proteins are therefore not solely bound by ionic forces to the isolated membranes from bovine caudate nucleus.Keywords
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