Interactions among Hageman factor, plasma prekallikrein, high molecular weight kininogen, and plasma thromboplastin antecedent.

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Abstract
To investigate the earliest steps of the intrinsic clotting pathway, [human] Hageman factor (factor XII) was exposed to Sephadex gels to which ellagic acid had been adsorbed. Hageman factor was then separated from the gels and studied in the fluid phase. Sephadex-ellagic acid-exposed Hageman factor, whether purified or in plasma, activated plasma thromboplastin antecedent, but only when high MW kininogen was present. Without plasma prekallikrein, maximal activation of plasma thromboplastin antecedent was slightly delayed in plasma, a delay not observed with similarly treated purified Hageman factor. High MW kininogen was needed for expression of Hageman factor''s clot-promoting properties and plasma prekallikrein played a minor role in the interaction of ellagic acid-treated Hageman factor and plasma thromboplastin antecedent.