Substrates for rumen β-glucosidase

Publisher Website
Google Scholar
Abstract
[beta]-Glucosidase activity of cell-free extracts of microorganisms from sheep rumen was measured with o-nitrophenyl [beta]-glucoside, cellobiose and salicin as substrates. Saligenin liberated from salicin increases with substrate concentration even when the reducing substances liberated reach a constant value and decrease. Cellobiose acts chiefly as a competitive inhibitor of the hydrolysis of o-nitrophenyl [beta]-glucoside; salicin, glucose and glucono-lactone are essentially competitive inhibitors.