Purification of dihydropteridine reductase from human platelets
- 1 January 1981
- journal article
- research article
- Published by Wiley in Journal of Neuroscience Research
- Vol. 6 (2) , 193-201
- https://doi.org/10.1002/jnr.490060205
Abstract
Dihydropteridine reductase was purified approximately 1,700-fold from human outdated blood platelets. Two forms of the enzyme, A and B, were resolved. They have the same Km values for 2-amino-6,7,-dimethyl-4-hydroxydihydropteridine (46 μml;M vs 49 μml;M), but the A form has a Km for NADH that is two times higher than that of the B form (20 μml;M vs 9 μml;M).Keywords
This publication has 18 references indexed in Scilit:
- LOW CSF HYDROXYLASE COFACTOR (TETRAHYDROBIOPTERIN) LEVELS IN INHERITED DYSTONIAThe Lancet, 1979
- Human dihydropteridine reductase a method for the measurement of activity in cultured cells, and its application to malignant hyperphenylalaninemiaClinica Chimica Acta; International Journal of Clinical Chemistry, 1979
- Hydroxylase Cofactor Activity in Cerebrospinal Fluid of Normal Subjects and Patients with Parkinson's DiseaseScience, 1979
- Purification of dihydropterin reductase using immobilized Cibacron BlueCanadian Journal of Biochemistry, 1979
- A Naphthoquinone Adsorbent for Affinity Chromatography of Human Dihydropteridine ReductaseEuropean Journal of Biochemistry, 1978
- Biogenic Amine Synthesis Defect in Dihydropteridine Reductase DeficiencyScience, 1977
- Purification and characterization of beef liver dihydrofolate reductaseArchives of Biochemistry and Biophysics, 1976
- Phenylketonuria Due to a Deficiency of Dihydropteridine ReductaseNew England Journal of Medicine, 1975
- Biosynthesis of catecholamines in organotypic cultures of the peripheral autonomic nervous system: Modifications by biopterin and other agentsJournal of Neurobiology, 1975
- Dihydropteridine ReductaseEuropean Journal of Biochemistry, 1969