Chromium binding by calf thymus nuclei and effects on chromatin

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Abstract
No binding of chromium was detected after incubation of calf thymus nuclei with hexavalent chromium up to 0.5 mM. Chromium was readily taken up and tightly bound after incubation with trivalent chromium. In a DNA‐filter binding assay, increasing amounts of chromium and DNA were bound with increasing chromium trichloride concentrations incubated with the nuclei. Treatment with proteinase K abolished the increase in DNA retention induced by trivalent chromium. It is concluded that trivalent chromium is the ultimate genetoxic agent after chromate uptake by living cells.