Enzyme Immunoassay of Testosterone Using the Testosterone-Glucoamylase Complex
- 1 July 1976
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Biochemistry
- Vol. 80 (1) , 191-194
- https://doi.org/10.1093/oxfordjournals.jbchem.a131252
Abstract
A highly sensitive, reproducible method was established for enzyme-coupled immunoassay of testosterone, involving coupling of testosterone to glucoamylase (EC 3.2.1.3) with a water-soluble coupling reagent carbodiimide. No enzyme activity was lost during this coupling procedure. The sensitivity of the method was comparable to that of competitive protein binding assay.This publication has 5 references indexed in Scilit:
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- Determination of Plasma Testosterone by the Use of Competitive Protein BindingJournal of Clinical Endocrinology & Metabolism, 1968
- The measurement of digitoxin in human serum by radioimmunoassayJournal of Clinical Investigation, 1968
- The Measurement of Pyridine Nucleotides by Enzymatic CyclingJournal of Biological Chemistry, 1961
- STEROID-PROTEIN CONJUGATES .2. PREPARATION AND CHARACTERIZATION OF CONJUGATES OF BOVINE SERUM ALBUMIN WITH PROGESTERONE, DEOXYCORTICOSTERONE, AND ESTRONE1959