Ku80: product of the XRCC5 gene and its role in DNA repair and V(D)J recombination
- 2 September 1994
- journal article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 265 (5177) , 1442-1445
- https://doi.org/10.1126/science.8073286
Abstract
The radiosensitive mutant xrs-6, derived from Chinese hamster ovary cells, is defective in DNA double-strand break repair and in ability to undergo V(D)J recombination. The human XRCC5 DNA repair gene, which complements this mutant, is shown here through genetic and biochemical evidence to be the 80-kilodalton subunit of the Ku protein. Ku binds to free double-stranded DNA ends and is the DNA-binding component of the DNA-dependent protein kinase. Thus, the Ku protein is involved in DNA repair and in V(D)J recombination, and these results may also indicate a role for the Ku-DNA-dependent protein kinase complex in those same processes.Keywords
This publication has 28 references indexed in Scilit:
- Gene mutation and V(D)J recombination in the radiosensitive irs linesMutagenesis, 1994
- Subchromosomal localization of a gene (XRCC5) involved in double strand break repair to the region 2q34-36Somatic Cell and Molecular Genetics, 1993
- VDJ recombinationImmunology Today, 1992
- On the mechanisms of ku protein binding to DNABiochemical and Biophysical Research Communications, 1992
- Genetic analysis of ionising radiation sensitive mutants of cultured mammalian cell linesMutation Research/DNA Repair, 1991
- The scid mutation in mice causes a general defect in DNA repairNature, 1990
- RAG-1 and RAG-2, Adjacent Genes That Synergistically Activate V(D)J RecombinationScience, 1990
- HeLa nuclear protein recognizing DNA termini and translocating on DNA forming a regular DNA-multimeric protein complexJournal of Molecular Biology, 1989
- The defect in murine severe combined immune deficiency: Joining of signal sequences but not coding segments in V(D)J recombinationCell, 1988
- The scid defect affects the final step of the immunoglobulin VDJ recombinase mechanismCell, 1988