Genomic sequences with homology to the P element of Drosophila melanogaster occur in the blowfly Lucilia cuprina.
- 15 November 1992
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 89 (22) , 10753-10757
- https://doi.org/10.1073/pnas.89.22.10753
Abstract
We have cloned two DNA elements (Lu-P1 and Lu-P2) from the Australian sheep blowfly Lucilia cuprina that are similar to the transposable P element of Drosophila melanogaster in both structure and sequence but have diverged from it and from each other considerably. Hybridization studies indicate that a third related element probably exists in another, as yet unsequenced, clone. Neither Lu-P1 nor Lu-P2 appears to be active in terms of mobility, and it is not known whether any transposition-competent copies of other related elements occur in the genome of the blowfly. However, the isolation of any P-like sequences from a species outside of the family Drosophilidae allows comparisons to be made of more widely divergent P-related elements than has been possible previously. We are unaware of any report of the presence of multiple P-like family members within a single species. The discovery of Lu-P1 and Lu-P2 in the blowfly fuels the possibility that similar elements may be widespread in insects, and perhaps in other orders of animals.Keywords
This publication has 25 references indexed in Scilit:
- Detection of specific sequences among DNA fragments separated by gel electrophoresisPublished by Elsevier ,2006
- Regulation of Drosophila P element transpositionTrends in Genetics, 1991
- Evidence for a common evolutionary origin of inverted repeat transposons in Drosophila and plants: hobo, activator, and Tam3Cell, 1991
- Insertional Mutagenesis of the Drosophila Genome with Single P ElementsScience, 1988
- Molecular evolution inDrosophila and the higher dipteraJournal of Molecular Evolution, 1984
- Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencingGene, 1984
- Isolation of the transposable maize controlling elements Ac and DsCell, 1983
- Structures of P transposable elements and their sites of insertion and excision in the Drosophila melanogaster genomeCell, 1983
- Chromosomal walking and jumping to isolate DNA from the Ace and rosy loci and the bithorax complex in Drosophila melanogasterJournal of Molecular Biology, 1983
- Genetic Transformation of Drosophila with Transposable Element VectorsScience, 1982