METASTASIS SPREAD FROM SYNGENEIC MURINE TUMORS - ESTABLISHMENT OF A TEST PROTOCOL FOR COMPARISONS BETWEEN ASCITES TUMORS AND THEIR PROGENITORS

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  • 1 January 1978
    • journal article
    • research article
    • Vol. 86  (3) , 231-239
Abstract
Two new MC[methylcholanthrene]-induced tumors, a sarcoma (MCB21-SS) and a carcinoma (MCB31-SC), were transformed into ascites form. When transplanted s.c. these ascites tumors grow as solid, quite undifferentiated tumors. (AS = ascites solid tumours). The metastasizibility of the AS tumors was compared with that of the parent tumors. In doing so, both the tail and the hind leg were used as transplantation sites. The tumors can be radically removed from both sites by amputation, which prolongs the survival time of the animals and permits metastases to grow into detectable sizes. As registered grossly and by microscopy, the AS tumors have a greater tendency of spread than the parent (SS/SC) tumors. MCB-21AS grows quicker than 21-SS and gives rise to more lymph node metastases. When transplanted to the tail the AS tumor also gives a higher incidence of lung metastases. No such difference was detected by leg-transplanted tumors. MCB31-SC did not produce any detectable metastases at all, while 31-AS, particularly from the tail, gave rise to numerous lymph node and lung metastases. There were no differences in tumor size or growth rate to account for this difference. Thus ascites conversion has changed the carcinoma MCB31-SC into an undifferentiated, metastasizing tumor, as detected by this procedure. The design of test protocols to detect metastasizibility is discussed.