The Location of Bound Lipid in the Lipovitellin Complex
- 31 July 1992
- journal article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 257 (5070) , 652-655
- https://doi.org/10.1126/science.1496377
Abstract
The location of the bound lipid in the soluble lipoprotein lipovitellin has been determined by neutron crystallographic techniques. With the use of the contrast variation method, whereby the crystals are soaked in different H 2 O-D 2 O mixtures, the lipid has been found to occupy a large cavity in the protein whose structure had previously been determined by x-ray crystallography. The lipid appears to be bound in the form of a bilayer with the major protein-lipid interactions being hydrophobic and with the lipid headgroups projecting into the bulk solvent and into a solvent-filled space in the cavity.Keywords
This publication has 10 references indexed in Scilit:
- Structure and Function of a Lipoprotein: LipovitellinAnnual Review of Biophysics, 1991
- Computer‐Generated Explanations in Project Management SystemsJournal of Computing in Civil Engineering, 1991
- Structure of the lamprey yolk lipid-protein complex lipovitellin-phosvitin at 2.8 Å resolutionJournal of Molecular Biology, 1988
- Low resolution structures of biological complexes studied by neutron scatteringEuropean Biophysics Journal, 1988
- Best density maps in low-resolution crystallography with contrast variationActa Crystallographica Section A Foundations of Crystallography, 1987
- Resolution and Linearity of Anger-Type Neutron-Position Detectors as Simulated with Different Signal Processing and OpticsIEEE Transactions on Nuclear Science, 1985
- Preparation of single crystals of a yolk lipoproteinJournal of Molecular Biology, 1984
- Scaling and phase-difference determination in solvent contrast variation experimentsJournal of Applied Crystallography, 1984
- Lipid domains in the crystalline lipovitellin/phosvitin complex: a phosphorus-31 and proton NMR studyBiochemistry, 1982
- Structural analysis of hydrated egg lecithin and cholesterol bilayers II. Neutron diffractionJournal of Molecular Biology, 1976