ANTIBIOTIC PRODUCTION OF HYPHAL FRACTIONS OF STREPTOMYCES GRISEUS .2. STREPTOMYCIN PRODUCTION OF DIFFERENT FRACTIONS OBTAINED BY DENSITY GRADIENT CENTRIFUGATION
- 1 January 1967
- journal article
- research article
- Vol. 15 (5) , 1010-+
Abstract
The streptomycin-producing activity (SPA) of hyphal fractions from washed mycelium of submerged cultures of S. griseus strain 52-1, as obtained by density gradient centrifugation, was in-vestigated. Activity of the various fractions differed strongly in in-tensity. The highest SPA was evident in the unfractionated mycelium. A synergistic effect upon SPA was found in the interaction of cultures of different ages, and a 55% increase in yield was obtained by mixing the 48- and 72-hr. cultures. A synergistic effect occurred in all combinations studied. By use of fractions obtained from 72 hr. mycelium for inoculation, differences in streptomycin production were noted. Some inoculum frac-tions yielded a greater amount of streptomycin (36%) than the unfractionated mycelialinoculum.This publication has 3 references indexed in Scilit:
- Isolation of different mycelial fractions from submerged culture of Streptomyces griseus strain by density gradient centrifugation.1967
- ANTIBIOTIC PRODUCTION OF HYPHAL FRACTIONS OF STREPTOMYCES GRISEUS .I. DETERMINATION OF STREPTOMYCIN PRODUCING ACTIVITY OF WASHED MYCELIUM IN SHORT-TERM INCUBATION1967
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951