Characteristics of DNA and Multiple rpoD Homologs of Microcystis(Synechocystis) Strains
- 1 October 1993
- journal article
- research article
- Published by Microbiology Society in International Journal of Systematic and Evolutionary Microbiology
- Vol. 43 (4) , 844-847
- https://doi.org/10.1099/00207713-43-4-844
Abstract
The base compositions of DNAs from nine Microcystis strains, as determined by high-performance liquid chromatography, were 41 to 42 mol% G+C. Chromosomal DNAs derived from these strains were found to be extremely resistant to many restriction endonucleases, and a restriction analysis revealed the presence of a dam-like methylase or both dam- and dcm-like methylases in all of the strains examined. Genomic Southern hybridization in which a synthetic oligonucleotide probe (rpoD probe) was used showed that members of the genus Microcystis might have multiple rpoD homologs, and the hybridization signal patterns observed with the DNAs of Microcystis aeruginosa strains were different from each other.Keywords
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