THE EFFECTS OF DIAZENE DICARBOXYLIC-ACID BIS-(N, N-DIMETHYLAMIDE) ON GLYCINE UPTAKE BY NEWBORN RENAL CORTEX

  • 1 January 1980
    • journal article
    • research article
    • Vol. 1  (2) , 161-169
Abstract
Glycine uptake by newborn rat renal cortical slices in the presence of 2 mM diamide remained unchanged from control values during incubation times up to 30 min. Longer exposure to diamide resulted in decreased net uptake. Kinetic analysis indicated a noncompetitive inhibitory effect of diamide on the low-affinity glycine uptake system; glycine uptake on the high-affinity system was obliterated. A similar analysis of the diamide effects on adult tissue indicated a noncompetitive inhibition of both the low- and high-affinity glycine transport systems. Diamide did not cause any demonstrable change in glycine efflux from newborn cortical slices. The decreased net uptake observed in newborn slices in the presence of diamide could evidently be explained solely on the basis of an effect on glycine entry. Simultaneous measurements of intracellular glutathione levels in the newborn tissue showed a lack of any direct relationship between the transport effects of diamide and its oxidative effect on reduced glutathione.

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