The assay of riboflavin in cereals and other products

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Abstract
The microbiological method (Snell and Strong) and the fluorimetric method (Najjar) were improved and modified with special reference to cereals and cereal products. In the microbiological method, the chief modifications were addition of xylose (1/1000) nicotinic and pantothenic acids to the medium (0.05 g./ml. medium). The presence of starch and fat stimulated the growth of Lactobacillus helveticus; it must be removed from the assay samples. Taka-diastase was unsuitable for the hydrolysis of starch since many samples appeared to contain appreciable amts. of riboflavin. The most convenient method of preparing an extract was heating in an autoclave at 15 lbs. pressure for 15 mins. with 0.25.N HCl. In the fluorimetric method, Superfiltrol was used as adsorbent simultaneously to purify and concentrate the riboflavin present in an already conc. cereal hydrolysate. Results obtained from a wide range of products by the 2 methods showed good agreement. The microbiological method is preferred as it is more rapid, and requires less material and no specialised apparatus.

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