The Rok Protein of Bacillus subtilis Represses Genes for Cell Surface and Extracellular Functions
Open Access
- 15 March 2005
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 187 (6) , 2010-2019
- https://doi.org/10.1128/jb.187.6.2010-2019.2005
Abstract
Rok is a repressor of the transcriptional activator ComK and is therefore an important regulator of competence in Bacillus subtilis (T. T. Hoa, P. Tortosa, M. Albano, and D. Dubnau, Mol. Microbiol. 43:15-26, 2002). To address the wider role of Rok in the physiology of B. subtilis, we have used a combination of transcriptional profiling, gel shift experiments, and the analysis of lacZ fusions. We demonstrate that Rok is a repressor of a family of genes that specify membrane-localized and secreted proteins, including a number of genes that encode products with antibiotic activity. We present evidence for the recent introduction of rok into the B. subtilis-Bacillus licheniformis-Bacilllus amyloliquefaciens group by horizontal transmission.Keywords
This publication has 41 references indexed in Scilit:
- The SapB morphogen is a lantibiotic-like peptide derived from the product of the developmental gene ramS in Streptomyces coelicolorProceedings of the National Academy of Sciences, 2004
- Proteomics of Protein Secretion by Bacillus subtilis : Separating the “Secrets” of the SecretomeMicrobiology and Molecular Biology Reviews, 2004
- Mechanism of Transcription Activation at the comG Promoter by the Competence Transcription Factor ComK of Bacillus subtilisJournal of Bacteriology, 2004
- TheBacillus subtilistransition state regulator AbrB binds to the −35 promoter region ofcomKFEMS Microbiology Letters, 2003
- Thiol-Disulfide Oxidoreductases Are Essential for the Production of the Lantibiotic Sublancin 168Journal of Biological Chemistry, 2002
- Whole-Genome Analysis of Genes Regulated by theBacillus subtilisCompetence Transcription Factor ComKJournal of Bacteriology, 2002
- The N-acetylmuramoyl-L-alanine amidase encoded by the Bacillus subtilis 168 prophage SPβMicrobiology, 1998
- comK encodes the competence transcription factor, the key regulatory protein for competence development in Bacillus subtilisMolecular Microbiology, 1995
- Biochemical and genetic characterization of a competence pheromone from B. subtilisCell, 1994
- Molecular cloning and sequence of comK, a gene required for genetic competence in Bacillus subtilisMolecular Microbiology, 1994