LEVELS OF OESTROGEN AND PROGESTERONE IN THE PLASMA OF THE COW DURING THE LAST MONTH OF PREGNANCY

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Abstract
The development of sensitive and accurate radioimmunoassay (RIA) techniques for the determination of ovarian hormones has made possible the study of their circulating levels during the oestrous cycle and pregnancy of many animals. Results for the levels of oestrogens and progesterone in the cyclic cow using RIA techniques have been reported by Henricks, Dickey & Hill (1971), for oestrogens by Glencross, Pope & Munro (1972) and for progesterone by Sprague, Hopwood, Niswender & Wiltbank (1971). Levels of oestrone during pregnancy, estimated by a fluorometric procedure, have been reported by Robinson, Baker, Anastassiadis & Common (1970), and using a protein-binding method Donaldson, Bassett & Thorburn (1970) reported maximum levels of progesterone (7–8 ng/ml) between 20 and 40 days pre partum falling to less than 1 ng/ml 2–3 days post partum. The present paper reports the levels of oestrogens and progesterone during the last month of pregnancy in five cows from a

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