Ligand‐transporter interaction in the AcrB multidrug efflux pump determined by fluorescence polarization assay
Open Access
- 25 September 2007
- journal article
- research article
- Published by Wiley in FEBS Letters
- Vol. 581 (25) , 4972-4976
- https://doi.org/10.1016/j.febslet.2007.09.035
Abstract
The AcrB of Escherichia coli pumps out a wide range of compounds, including most of the currently available antibiotics, and contributes significantly to the serious problem of multidrug resistance o...Keywords
This publication has 30 references indexed in Scilit:
- Characterization of the multidrug efflux regulator AcrR from Escherichia coliBiochemical and Biophysical Research Communications, 2007
- Multidrug-Binding Transcription Factor QacR Binds the Bivalent Aromatic Diamidines DB75 and DB359 in Multiple PositionsJournal of the American Chemical Society, 2007
- Drug Export Pathway of Multidrug Exporter AcrB Revealed by DARPin InhibitorsPLoS Biology, 2006
- Crystal structure of the multidrug efflux transporter AcrB at 3.1 Å resolution reveals the N-terminal region with conserved amino acidsJournal of Structural Biology, 2006
- Conformation of the AcrB Multidrug Efflux Pump in Mutants of the Putative Proton Relay PathwayJournal of Bacteriology, 2006
- Threonine-978 in the Transmembrane Segment of the Multidrug Efflux Pump AcrB of Escherichia coli Is Crucial for Drug Transport as a Probable Component of the Proton Relay NetworkJournal of Bacteriology, 2006
- Structural Asymmetry of AcrB Trimer Suggests a Peristaltic Pump MechanismScience, 2006
- A Periplasmic Drug-Binding Site of the AcrB Multidrug Efflux Pump: a Crystallographic and Site-Directed Mutagenesis StudyJournal of Bacteriology, 2005
- Structural Basis of Multiple Drug-Binding Capacity of the AcrB Multidrug Efflux PumpScience, 2003
- Application of fluorescence energy transfer and polarization to monitor Escherichia coli cAMP receptor protein and lac promoter interaction.Proceedings of the National Academy of Sciences, 1990