Binding of Thiostrepton to a Complex of 23‐S rRNA with Ribosomal Protein L11

Abstract
Thiostrepton binds with high affinity and with a 1:1 stoichiometry to a complex formed between Escherichia coli 23‐S ribosomal RNA and ribosomal protein L11 of E. coli or the homologous protein BM‐L11 of Bacillus megaterium. In the presence of T1 ribonuclease, protein BM‐L11 and thiostrepton protect from degradation a fragment of E. coli 23‐S RNA estimated to be about 50 nucleotides in length.