EFFECT OF CYTOCHALASIN B AND DI‐HYDROCYTOCHALASIN B ON INVASIVENESS OF ENTERO‐INVASIVE BACTERIA IN HEp‐2 CELL CULTURES
- 1 September 1984
- journal article
- research article
- Published by Wiley in Acta Pathologica Microbiologica Scandinavica Series B: Microbiology
- Vol. 92B (1-6) , 145-149
- https://doi.org/10.1111/j.1699-0463.1984.tb02809.x
Abstract
The effect of cytochalasin B (CB) and di‐hydrocytochalasin B (H2CB) on the invasiveness of Salmonella typhimurium, Shigella flexneri and Yersinia enterocolotica serotype 0:3 in HEp‐2 cell cultures was examined. The intra‐cellular and extra‐cellular bacteria were identified by a combination of Nomarski interference contrast microscopy and UV incident light microscopy applied on the same light microscope. Pre‐treatment of cells with CB and H2CB inhibited the uptake of S. typhimurium and S. flexneri in the HEp‐2 cell cultures. The effect was time and dose dependent. On the other hand, the drugs did not influence the invasiveness of Y. enterocolitica. The results indicate that activity of cellular actin micro‐filaments is essential for invasiveness of S. typhimurium and S. flexneri.Keywords
This publication has 28 references indexed in Scilit:
- ENDOCYTOSIS OF SALMONELLA TYPHIMURIUM 395 MS AND MR10 BY HELA CELLSActa Pathologica Microbiologica Scandinavica Section B Microbiology, 2009
- INVASIVENESS OF SALMONELLA TYPHIMURIUM IN HEp‐2 CELL CULTURES PREINFECTED WITH COXSACKIE B 1 VIRUSActa Pathologica Microbiologica Scandinavica Series B: Microbiology, 1984
- Inhibition of cytokinesis and altered contractile ring morphology induced by cytochalasins in synchronized PtK2 cellsExperimental Cell Research, 1981
- Dihydrocytochalasin B. Biological effects and binding to 3T3 cells.The Journal of cell biology, 1978
- Cytochalasin B binding sites and glucose transport carrier in human erythrocyte ghosts.Journal of Biological Chemistry, 1977
- Studies on the Pathogenicity ofYersinia enterocoliticaMicrobiology and Immunology, 1977
- Binding of cytochalasin B to a red cell membrane proteinBiochemical and Biophysical Research Communications, 1974
- Actin And Myosin And Cell MovemenCRC Critical Reviews in Biochemistry, 1974
- Invasion of HeLa Cells by Salmonella typhimurium: A Model for Study of Invasiveness of SalmonellaThe Journal of Infectious Diseases, 1973
- EXPERIMENTAL MODELS IN THE INVESTIGATION OF THE VIRULENCE OF DYSENTERY BACILLI AND ESCHERICHIA COLI*Annals of the New York Academy of Sciences, 1971