Comparison of two methods of genetic exchange in determination of the genetic locus of the structural gene for Pseudomonas aeruginosa elastase

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Abstract
A mutation, lasA1, that results in the synthesis of enzymatically inactive elastase protein, was mapped by R68.45 plasmid-mediated conjugation and transposon-facilitated recombination to .apprx. 75 min on the P. aeruginosa PAO chromosome. Since immunologically cross-reactive material is produced, the mutation presumably arose within the elastase structural gene.