The Determination of Scopoletin in Environmental Tobacco Smoke by High-Performance Liquid Chromatography

Publisher Website
Google Scholar
Abstract
A high-performance liquid chromatographic (HPLC) method was developed for the quantification of scopoletin in environmental tobacco smoke (ETS) for use as an indicator of tobacco smoke in indoor air. Samples were collected on 1 μm pore size Fluoropore membrane filters and extracted with methanol (MeOH). This single extract was used for four analyses including scopoletin after dilution with 1% aqueous acetic acid (HAc). Scopoletin was determined on an octadecyl silane, silica-based column with fluorescence detection. A mobile phase gradient of 1% HAc and 99% acetonitrile + 1% glacial acetic acid was used. The method was reproducible with a relative standard deviation of 6.4%. Percent recovery was 98%. Analyses of smoke from oak leaves indicated the presence of scopoletin, but it was not detected in fireplace smoke. A short sampling time of one h was required and the procedure was capable of detecting < 3 ng m−3.