Isolation of a cDNA clone for the human muscle specific carbonic anhydrase, CAIII
- 1 July 1985
- journal article
- Published by Wiley in Annals of Human Genetics
- Vol. 49 (3) , 241-251
- https://doi.org/10.1111/j.1469-1809.1985.tb01698.x
Abstract
The molecular cloning of cDNA for the human muscle specific carbonic anhydrase CAIII is described. The recombinant was isolated from a human muscle cDNA library prepared in the expression vector lambda gt11, and was characterized by hybridization selection and immunoprecipitation. A comparison of insert cDNA and mRNA sizes suggests that the cDNA is full length and includes extensive untranslated sequences. Preliminary sequence data have confirmed the authenticity of this clone and Southern blotting of human and rodent DNA indicates that it will be a useful probe in the analysis of somatic cell hybrids.Keywords
This publication has 21 references indexed in Scilit:
- Molecular cloning of a cDNA for human alpha-L-fucosidase.Proceedings of the National Academy of Sciences, 1985
- Chromogenic Immuno detection of Human Serum Albumin and α-l-Fucosidase Clones in a Human Hepatoma cDNA Expression LibraryDNA, 1984
- Origins and Molecular Evolution of the Carbonic Anhydrase IsozymesaAnnals of the New York Academy of Sciences, 1984
- The nucleotide sequence and derived amino acid sequence of cDNA coding for mouse carbonic anhydrase IIGene, 1983
- A simple and very efficient method for generating cDNA librariesGene, 1983
- Buffer gradient gels and 35S label as an aid to rapid DNA sequence determination.Proceedings of the National Academy of Sciences, 1983
- Distribution of CAIII in fetal and adult human tissueBiochemical Genetics, 1980
- Characterization of human carbonic anhydrase III from skeletal muscleBiochemical Genetics, 1979
- Evolution of mammalian carbonic anhydrase loci by tandem duplication: Close linkage of Car-1 and Car-2 to the centromere region of chromosome 3 of the mouseBiochemical Genetics, 1976
- Morphogenesis and Regulation in Spite of Continued Mitotic Inhibition in Xenopus EmbryosNature, 1973